One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins
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One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs.
We describe a one-step affinity method for purifying ribosomes from the budding yeast Saccharomyces cerevisiae. Extracts from yeast strains expressing only C-terminally tagged Rpl25 protein or overexpressing this protein in the presence of endogenous Rpl25p were used as the starling materials. The purification was specific for tagged 60S subunits, and resulted in the copurification of 80S subun...
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40. M. E. and Dickson, R. B. (1993) Cancer Res. 53, 1409-141 5 McLeskey, S. W., Kurebayashi, J.$ Honig, S. F., Zwiebel, J., Iippman, M. E., Dickson. R. H. and Kern, F. G. (1993) Cancer Res. 53,2168-2177 Kurebayashi, J., McLeskey, S. W., Johnson, M. D.. Lippman. M. E., Dickson, R. H. and Kern. F. G. (1993) Cancer Res. 53,2178-2187 Dickson, H. €3. (1993) Cancer Res. 53,873-877 38. Shi, Y. E., Tor...
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In budding yeast and higher eukaryotic genomes, there are, respectively, 50 and up to 400 or more distinct genes that encode for ubiquitin-ligases, and approximately 15-90 genes that encode for ubiquitin isopeptidases (TM and RJD, Semple et al., 2003). This puts ubiquitylation on par with phosphorylation as the most common reversible posttranslational modifications in eukaryotic cells. A key ch...
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Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...
متن کاملEfficient, ultra-high-affinity chromatography in a one-step purification of complex proteins.
Protein purification is an essential primary step in numerous biological studies. It is particularly significant for the rapidly emerging high-throughput fields, such as proteomics, interactomics, and drug discovery. Moreover, purifications for structural and industrial applications should meet the requirement of high yield, high purity, and high activity (HHH). It is, therefore, highly desirab...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2018
ISSN: 1475-2859
DOI: 10.1186/s12934-018-1039-z